FCN1 Chemi-Luminescent ELISA Kit (Rat) (OKCD03452)

Catalog No: OKCD03452

Size:96WELLS

Rat FCN1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	90-104%	81-103%	87-101%	84-94%
  EDTA Plasma (n=5)	79-95%	91-101%	81-88%	96-104%
  Heparin Plasma (n=5)	78-98%	78-89%	89-101%	84-98%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Ficolin 1 (FCN1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Ficolin 1 (FCN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Ficolin 1 (FCN1) level in the sample or standard.
• ELISA Kit Range: 68.6-50,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	79-94	91
  EDTA Plasma (n=5)	91-98	94
  Heparin Plasma (n=5)	82-96	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ficolin 1 (FCN1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ficolin 1 (FCN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Fcn1 Microplate: 96 Wells (12 x 8 Well strips)
  • Fcn1 Lyophilized Standard: 2
  • 100X Fcn1 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.781 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Fcn1
• Alias Symbols: Collagen/fibrinogen domain-containing protein 1;Ficolin-A;Ficolin-alpha;M-ficolin.
• NCBI Gene Id: 83517
• Protein Name: Ficolin-1
• Description of Target: Extracellular lectin functioning as a pattern-recognition receptor in innate immunity. Binds the sugar moieties of pathogen-associated molecular patterns (PAMPs) displayed on microbes and activates the lectin pathway of the complement system. May also activate monocytes through a G protein-coupled receptor, FFAR2, inducing the secretion of interleukin-8/IL-8. Binds preferentially to 9-O-acetylated 2-6-linked sialic acid derivatives and to various glycans containing sialic acid engaged in a 2-3 linkage (By similarity).
• Uniprot ID: Q9WTS8
• Protein Accession #: NP_112638
• Nucleotide Accession #: NM_031348