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Catalog No: OKBB01003
Size:96 wells
Price: $629.00
SKU
OKBB01003
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva's mouse FCRN ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for FCRN has been precoated onto 96-well plates. Standards(Expression system for standard: NS0, Immunogen sequence: S22-S297) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FCRN is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse FCRN amount of sample captured in plate.
ELISA Kit Range93.7 pg/ml - 6,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)1321019242314311172272
Standard deviation5.2871.33118.727.5799.41134.04
CV(%)474.95.38.95.9
ELISA Kit Component
ComponentAmount
96-Well Plate Pre-coated with Anti-Mouse FCGRT Antibody96 Wells (12 x 8 Well Strips)
Lyophilized Recombinant Mouse FCGRT Standard2 x 10 ng
100X Biotinylated Anti-Mouse FCGRT Antibody1 x 130 uL
100X Avidin-Biotin Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Additional Information

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

LotsLot1 (pg/ml)Lot2 (pg/ml)Lot3 (pg/ml)Lot4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11321231301221264.323.4%
Sample 21019107091595398959.656%
Sample 324232361220725202377113.74.7%

*number of samples for each test n=16.

::
Standard Number87654321
Concentration (pg/ml)093.7187.5375750150030006000
O.D.0.0750.1170.1700.2360.3850.7441.3372.224
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample Typecell culture supernates, serum, plasma (heparin, EDTA) andcell lysates
Sensitivity<10 pg/ml
SpecificityNatural and recombinant mouse FCRN
ImmunogenExpression system for standard: NS0; Immunogen sequence: S22-S297
DescriptionFor quantitative detection of mouse FCRN in cell culture supernatants, serum, plasma(heparin, EDTA) and cell lysates.
Gene SymbolFCGRT
Gene Full NameFc fragment of IgG receptor and transporter
Alias SymbolsFCGRT, FcRn alpha chain, FcRn, IgG Gc receptor, Neonatal Fc receptor, Q61559
NCBI Gene Id14132
Protein NameIgG receptor FcRn large subunit p51
Description of TargetThe neonatal Fc receptor (FcRn), also known as the Brambell receptor, is a protein that in humans is encoded by the FCGRT gene. The neonatal Fc receptor is an Fc receptor which is similar in structure to the MHC class I molecule and also associates with beta-2-microglobulin. It was first discovered in rodents as a unique receptor capable of transporting IgG from mother's milk across the epithelium of newborn rodent's gut into the newborn's bloodstream. Further studies revealed a similar receptor in humans, leading to the naming as a neonatal Fc receptor. In humans, however, it is found in the placenta to help facilitate transport of mother's IgG to the growing fetus and it has also been shown to play a role in monitoring IgG turnover. Neonatal Fc receptor expression is up-regulated by the proinflammatory cytokine,TNF-α, and down-regulated by IFN-γ.
Uniprot IDQ61559
Protein Accession #NP_034319.2
Nucleotide Accession #NM_010189.3
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