FCGR3A/FCGR3B Antibody : PE (OATA00311)

Catalog No: OATA00311

Human peripheral blood lymphocytes were stained with 5 uL (0.5 ug) PE Anti-Human CD16 (OATA00311) (solid line) or 0.5 ug PE Mouse IgG1 isotype control (dashed line).

Product Info

• Tested Species Reactivity: Human
• Product Format: Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
• Clonality: Monoclonal
• Clone: 3G8
• Isotype: Mouse IgG1, k
• Host: Mouse
• Conjugation: PE: Phycoerythrin
• Application: FC
  Excitation Laser: Blue (488 nm), Green (532 nm), Yellow-Green (561 nm)
  Emmision: 578 nm
• Reconstitution and Storage: 2-8C protected from light
• Specificity: CD16
• Application Info: This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 uL per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells.
• Reference:
  Fleit HB, Wright SD, Unkeless JC. 1982. Proc Natl Acad Sci U S A. May;79(10):3275-3279.
  Windebank KP, Abraham RT, Powis G, Olsen RA, Barna TJ, Leibson PJ. 1988. J Immunol. Dec 1;141(11):3951-3957.
  Fleit HB. 1991. Clin Immunol Immunopathol. May;59(2):222-235.
  Wirthmueller U, Kurosaki T, Murakami MS, Ravetch JV. 1992. J Exp Med. May 1;175(5):1381-1390.

Target Info

• Gene Symbol: FCGR3A, FCGR3B
• Gene Full Name: Fc fragment of IgG receptor IIIa
• Alias Symbols: CD16, FCG3, CD16A, FCGR3, IGFR3, IMD20, FCR-10, FCRIII, FCGRIII, FCRIIIA
• NCBI Gene Id: 2214; 2215
• Protein Name: low affinity immunoglobulin gamma Fc region receptor III-A
• Description of Target: This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: P08637, O75015
• Protein Accession #: NP_000560.6
• Nucleotide Accession #: NM_000569.7