FCGR2A ELISA Kit (Human) (OKCD00781)

Catalog No: OKCD00781

Size:96WELLS

Human FCGR2A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	87-97%	89-97%	86-101%	97-105%
  EDTA plasma(n=5)	98-105%	92-99%	95-103%	80-91%
  heparin plasma(n=5)	82-91%	96-104%	95-102%	80-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 32 (CD32). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 32 (CD32). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 32 (CD32), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cluster Of Differentiation 32 (CD32) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 46.88-3,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	94-103	97
  EDTA plasma(n=5)	91-101	95
  heparin plasma(n=5)	78-96	88

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation 32 (CD32) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation 32 (CD32) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-FCGR2A Microplate: 96 Wells (12 x 8 Well Strips)
  • FCGR2A Lyophilized Standard: 2 x 2 ng
  • 100X Biotinylated FCGR2A Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 17.35 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Receptor II For The Fc Region Of Immunoglobulin G (FcgRII). No significant cross-reactivity or interference between Receptor II For The Fc Region Of Immunoglobulin G (FcgRII) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: FCGR2A
• Gene Full Name: Fc fragment of IgG receptor IIa
• Alias Symbols: CD32;CD32A;CDw32;Fc fragment of IgG, low affinity IIa, receptor (CD32);Fc gamma receptor RIIa3;FCG2;Fc-gamma RII-a;fc-gamma-RIIa;FcGR;FCGR2;FCGR2A1;fcRII-a;IGFR2;igG Fc receptor II-a;Immunoglobulin G Fc receptor II;low affinity immunoglobulin gamma Fc region receptor II-a.
• NCBI Gene Id: 2212
• Protein Name: Low affinity immunoglobulin gamma Fc region receptor II-a
• Description of Target: Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens.
• Uniprot ID: P12318
• Protein Accession #: NP_001129691
• Nucleotide Accession #: NM_001136219.1
• Protein Size (# AA): 317