FCER2 ELISA Kit (Human) (OKCD07509)

Catalog No: OKCD07509

Size:96WELLS

FCER2 ELISA Kit (Human) (OKCD07509) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 23 (CD23). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 23 (CD23). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 23 (CD23), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cluster Of Differentiation 23 (CD23) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation 23 (CD23) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation 23 (CD23) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • FCER2 Microplate: 96 Wells (12 x 8 Well strips)
  • FCER2 Lyophilized Standard: 2
  • 100X FCER2 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.115ng/mL

Target Info

• Gene Symbol: FCER2
• Gene Full Name: Fc fragment of IgE receptor II
• Alias Symbols: BLAST-2;CD23;CD23 antigen;CD23A;CLEC4J;C-type lectin domain family 4 member J;C-type lectin domain family 4, member J;Fc epsilon receptor II;Fc fragment of IgE, low affinity II, receptor for (CD23);FCE2;fc-epsilon-RII;IGEBF;immunoglobulin E-binding factor;immunoglobulin epsilon-chain;low affinity immunoglobulin epsilon Fc receptor;lymphocyte IgE receptor.
• NCBI Gene Id: 2208
• Protein Name: Low affinity immunoglobulin epsilon Fc receptor
• Description of Target: Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).
• Uniprot ID: P06734
• Protein Accession #: NP_001193948.2
• Nucleotide Accession #: NM_001207019.2