FCER1A ELISA Kit (Rat) (OKEH05962)

Catalog No: OKEH05962

Size:96T

Rat FCER1A ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Fcer1a ELISA Kit (Rat) (OKEH05962) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Fcer1a has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Fcer1a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fcer1a captured in well.
• ELISA Kit Range: 78-5000pg/ml
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 80%
• ELISA Kit Reproducibility: Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
  Mean Intra-Assay Precision - <4.4%, n = 20
  Mean Inter-Assay Precision - <6.3%, n = 20
• ELISA Kit Component:
  • FCER1A Microplate: 96 Wells (12 x 8 Well strips)
  • FCER1A Lyophilized Standard: 2
  • 100X Biotinylated FCER1A Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 40.8 pg/mL
• Specificity: Natural and recombinant Rat High affinity immunoglobulin epsilon receptor subunit alpha
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Fcer1a
• Gene Full Name: Fc fragment of IgE receptor Ia
• Alias Symbols: alpha polypeptide;Fc fragment of IgE, high affinity I, receptor for;Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide;Fc receptor, IgE, high affinity I, alpha polypeptide;fc-epsilon RI-alpha;fcERI;high affinity immunoglobulin epsilon receptor subunit alpha;igE Fc receptor subunit alpha;Iger01;RATIGER01.
• NCBI Gene Id: 25047
• Protein Name: P12371
• Description of Target: High affinity immunoglobulin epsilon receptor subunit alpha
• Uniprot ID: https://www.uniprot.org/uniprot/P12371
• Protein Accession #: NP_036856.1
• Nucleotide Accession #: NM_012724.4
• Protein Size (# AA): 245