FCER1A ELISA Kit (Mouse) (OKCD07508)

Catalog No: OKCD07508

Size:96WELLS

FCER1A ELISA Kit (Mouse) (OKCD07508) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor I For The Fc Region Of Immunoglobulin E (FceRI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Receptor I For The Fc Region Of Immunoglobulin E (FceRI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Receptor I For The Fc Region Of Immunoglobulin E (FceRI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Receptor I For The Fc Region Of Immunoglobulin E (FceRI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Receptor I For The Fc Region Of Immunoglobulin E (FceRI) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Receptor I For The Fc Region Of Immunoglobulin E (FceRI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • FCER1A Microplate: 96 Wells (12 x 8 Well strips)
  • FCER1A Lyophilized Standard: 2
  • 100X FCER1A HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.057ng/mL

Target Info

• Gene Symbol: Fcer1a
• Gene Full Name: Fc receptor, IgE, high affinity I, alpha polypeptide
• Alias Symbols: FcE;Fce1;Fce1a;fc-epsilon RI-alpha;fcepsilonri;FcERI;Fcr-;Fcr-5;high affinity immunoglobulin epsilon receptor subunit alpha;igE Fc receptor subunit alpha.
• NCBI Gene Id: 14125
• Protein Name: High affinity immunoglobulin epsilon receptor subunit alpha
• Description of Target: Binds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines.
• Uniprot ID: P20489
• Protein Accession #: NP_034314.2
• Nucleotide Accession #: NM_010184.2