FBXW2 Antibody (OAEB02353)

Catalog No: OAEB02353 (Formerly GWB-CDAB9F)

Size:100UG

OAEB02353 staining (0.3ug/ml) of Human Kidney lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

OAEB02353 (3ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Porcine|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB, IHC
• Additional Information: Reactivity Info: Tested: Human; Expected: Human, Mouse
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: FBXW2
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Mouse: 92%
• Peptide Sequence: C-KRGSSFLAGEHPG
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:128000.
  Western Blot: Approx 48kDa band observed in Human Kidney lysates (calculated MW of 48.0kDa according to NP_036296.1). Recommended concentration: 0.3-1.5ug/ml. A stronger band of unknown identity was also consistently observed at appr. 30kDa - both bands were successfully blocked by incubation with the immunising peptide.
  Immunohistochemistry: In paraffin embedded Human Kidney, shows cytoplasmic staining of epithelial cells in some renal tubules. Recommended concentration, 2-3ug/ml.
• Reference: * Winston JT, Koepp DM, Zhu C, Elledge SJ, Harper JW.; A family of mammalian F-box proteins.; Curr Biol. 1999 Oct 21;9(20):1180-2.; Pubmed 10531037

Target Info

• Gene Symbol: FBXW2
• Gene Full Name: F-box and WD repeat domain containing 2
• Alias Symbols: F-box and WD-40 domain protein 2;F-box and WD-40 domain-containing protein 2;F-box/WD repeat-containing protein 2;FBW2;Fwd2;Md6.
• NCBI Gene Id: 26190
• Protein Name: F-box/WD repeat-containing protein 2 Ensembl ENSP00000398662
• Uniprot ID: Q4VXH1
• Protein Accession #: NP_036296.1
• Molecular Weight: 51.5