FBXO32 ELISA Kit (Mouse) (OKEH07775)

Catalog No: OKEH07775

Size:96T

Mouse FBXO32 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology FBXO32 ELISA Kit (Mouse) (OKEH07775) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FBXO32 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FBXO32 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FBXO32 captured in well.
• ELISA Kit Range: 15.6-1000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=3.8% (n = 20)
  Mean Inter-assay CV%: <=6.1% (n = 20)
• ELISA Kit Component:
  • FBXO32 Microplate: 96 Wells (12 x 8 Well strips)
  • FBXO32 Lyophilized Standard: 2
  • 100X Biotinylated FBXO32 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.
  Subcellular Location: Cytoplasm Nucleus Shuttles between cytoplasm and the nucleus.
  Unigene: Mm.25683
  SMR: Q9CPU7
  String: 10090.ENSMUSP00000022986
  KEGG: mmu:67731
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 7.86pg/mL
• Specificity: Natural and recombinant Mouse F-box only protein 32
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Fbxo32
• Gene Full Name: F-box protein 32
• Alias Symbols: 4833442G10Rik;AI430017;ATR;atro;atrogin 1;Atrogin-1;ATROGIN1;F-box only protein 32;Gm20361;MAFb;MAFbx;muscle atrophy F-box protein.
• NCBI Gene Id: 67731
• Protein Name: Q9CPU7
• Description of Target: F-box only protein 32
• Uniprot ID: https://www.uniprot.org/uniprot/Q9CPU7
• Protein Accession #: NP_080622.1
• Nucleotide Accession #: NM_026346.3