FBXO28 Antibody (OAGA05897)

Catalog No: OAGA05897

Size:100 ul

Sample (30 ug of whole cell lysate)
A: A549
B: H1299
10% SDS PAGE
diluted at 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FBXO28 antibody at 1:500 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human FBXO28. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.76 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: FBXO28
• Gene Full Name: F-box protein 28
• Alias Symbols: Fbx28, CENP-30
• NCBI Gene Id: 23219
• Protein Name: F-box only protein 28
• Description of Target: Members of the F-box protein family, such as FBXO28, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008]
• Uniprot ID: Q9NVF7
• Protein Accession #: NP_001129587.1
• Nucleotide Accession #: NM_001136115.2