FBXL2 Antibody - C-terminal region (OAAB02444)

Catalog No: OAAB02444

Size:400ul

FBXL2 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FBXL2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

FBXL2 Antibody (C-term) (Cat. #OAAB02444) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Mouse
• Product Format: Liquid.PBS with 0.09% (W/V) sodium azide
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC-P, FC, ELISA
• Additional Information: Cellular Location: Membrane; Lipid-anchor.
  Tissue Location: Expressed in brain, heart, kidney, liver, lung, pancreas and placenta.
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This FBXL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-395 amino acids from the C-terminal region of human FBXL2.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Varies by lot. See vial for concentration.
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000~1:2000
  IHC-P: 1:50~100
  FC: 1:10~50
  Optimal dilutions should be determined by the end user.
• Reference: Ye, J. PLoS Pathog. 3 (8), E108 (2007)
  Ilyin, G.P., et al. Genomics 67(1):40-47(2000)
  Winston, J.T., et al. Curr. Biol. 9(20):1180-1182(1999)
• Publications:

  Crystal structure and interaction studies of the human FBxo3 ApaG domain. FEBS J. 283, 2091-101 (2016). 27010866

Target Info

• Gene Symbol: FBXL2
• Alias Symbols: FBL2, FBL3
• NCBI Gene Id: 25827
• Protein Name: F-box/LRR-repeat protein 2
• Description of Target: FBXL2 encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains 12 tandem leucine-rich repeats.
• Uniprot ID: Q9UKC9, Q8BH16
• Protein Accession #: NP_001165184.1
• Molecular Weight: 47 kDa