FBLIM1 Antibody - C-terminal region (OAAB09001)

Catalog No: OAAB09001

Size:400ul

FBLIM1 Antibody (C-term) (Cat. #OAAB09001) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the FBLIM1 antibody detected the FBLIM1 protein (arrow).

FBLIM1 Antibody (C-term) (Cat. #OAAB09001)immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FBLIM1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC
• Additional Information: Precautions: FBLIM1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Serves as an anchoring site for cell-ECM adhesion proteins and filamin-containing actin filaments. Is implicated in cell shape modulation (spreading) and motility. May participate in the regulation of filamin-mediated cross-linking and stabilization of actin filaments. May also regulate the assembly of filamin- containing signaling complexes that control actin assembly Promotes dissociation of FLNA from ITGB3 and ITGB7. Promotes activation of integrins and regulates integrin-mediated cell-cell adhesion
  Cellular Location: Cytoplasm, cell cortex. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Note=Associated with actin stress fiber at cell-ECM focal adhesion sites. Isoform 1 and isoform 3 are recruited and localized at actin stress fibers and clustered at cell-EMC adhesion sites through interaction with PLEKHC1. Isoform 2 is localized at actin stress fibers
  Tissue Location: Isoform 1 and isoform 3 are expressed in heart, kidney, lung, pancreas, placenta and platelets. Isoform 2 is expressed in brain, heart, kidney, lung, pancreas, placenta, skeletal muscle and platelets
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This FBLIM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 294-323 amino acids from the C-terminal region of human FBLIM1.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50

Target Info

• Gene Symbol: FBLIM1
• Alias Symbols: CAL, FBLP1, FBLP-1
• NCBI Gene Id: 54751
• Protein Name: Filamin-binding LIM protein 1
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q8WUP2
• Protein Accession #: NP_001019386.1
• Molecular Weight: 40670 Da