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Catalog No: OKBB01480
Size:96 wells
Price: $675.00
SKU
OKBB01480
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva's human FASL ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FASL has been precoated onto 96-well plates. Standards(CHO, P134-L281) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FASL is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human FASL amount of sample captured in plate.
ELISA Kit Range15.6 pg/ml - 1,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)3618643734189419
Standard deviation2.199.6728.842.112.4733.1
CV(%)6.15.26.66.26.67.9
ELISA Kit Component
ComponentAmount
96-Well Plate Pre-coated with Anti-Human FASLG Antibody96 Wells (12 x 8 Well Strips)
Lyophilized Recombinant Human FASLG Standard2 x 10 ng
100X Biotinylated Anti-Human FASLG Antibody1 x 130 uL
100X Avidin-Biotin Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Additional Information

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

LotsLot1 (pg/ml)Lot2 (pg/ml)Lot3 (pg/ml)Lot4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 136414134383.088.1%
Sample 21861971921981934.762.4%
Sample 343749544650747130.216.4%

*number of samples for each test n=16.

::
Standard Number87654321
Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0080.1140.1960.3790.7401.3712.1212.693
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample Typecell culture supernates, serum and plasma (heparin, EDTA, citrate)
Sensitivity<2 pg/ml
SpecificityNatural and recombinant human FASL
ImmunogenExpression system for standard: CHO; Immunogen sequence: P134-L281
DescriptionThe Quick version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Human Fas Ligand/FASLG with <2pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernatants, serum and plasma(heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
Gene SymbolFASLG
Gene Full NameTumor necrosis factor ligand superfamily member 6
Alias SymbolsTumor necrosis factor ligand superfamily member 6;Apoptosis antigen ligand;APTL;CD95 ligand;CD95-L;Fas antigen ligand;Fas ligand;FasL;CD178;Tumor necrosis factor ligand superfamily member 6, membrane form;Tumor necrosis factor ligand superfamily member 6, soluble form;Receptor-binding FasL ectodomain;Soluble Fas ligand;sFasL;ADAM10-processed FasL form;APL;FasL intracellular domain;FasL ICD;SPPL2A-processed FasL form;SPA;FASLG;APT1LG1, CD95L, FASL, TNFSF6;
NCBI Gene Id356
Protein NameTumor necrosis factor ligand superfamily member 6
Description of TargetFas ligand(FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor(TNF) family. Its binding with its receptor induces apoptosis. The human FASL gene consists of approximately 8 kb and is split into 4 exons. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Fas ligand or FasL is a homotrimeric type II transmembrane protein. It signals through trimerization of FasR, which spans the membrane of the "target" cell. This trimerization usually leads to apoptosis, or cell death. Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external matrix metalloproteinase MMP-7.
Uniprot IDP48023
Protein Accession #NP_000630.1
Nucleotide Accession #NM_000639.2
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