FASLG Antibody (OAEE00681)

Catalog No: OAEE00681

Size:100T

Surface staining of CD178 in CD178-transfected L5178Y cells with anti-CD178 (NOK-1) PE.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: NOK-1
• Isotype: IgG1
• Host: Mouse
• Conjugation: R-PE
• Application: Flow cytometry
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: L5178Y mouse T lymphoma cells expressing recombinant human CD178
• Purification: Purified by size-exclusion chromatography
• Concentration: 10 ul / test
• Specificity: The mouse monoclonal antibody NOK-1 recognizes CD178 / Fas-L, an approximately 40 kDa transmembrane glycoprotein expressed on neutrophils, monocytes, and activated T and NK cells.
  HCDM Workshop: VII 70322
• Formulation: PE
• Reference: Kayagaki N, Kawasaki A, Ebata T, Ohmoto H, Ikeda S, Inoue S, Yoshino K, Okumura K, Yagita H: Metalloproteinase-mediated release of human Fas ligand. J Exp Med. 1995 Dec 1;182(6):1777-83.
  Ji J, Chen JJ, Braciale VL, Cloyd MW: Apoptosis induced in HIV-1-exposed, resting CD4+ T cells subsequent to signaling through homing receptors is Fas/Fas ligand-mediated. J Leukoc Biol. 2007 Jan;81(1):297-305.
  Chopin D, Barei-Moniri R, Maillé P, Le Frère-Belda MA, Muscatelli-Groux B, Merendino N, Lecerf L, Stoppacciaro A, Velotti F: Human urinary bladder transitional cell carcinomas acquire the functional Fas ligand during tumor progression. Am J Pathol. 2003 Apr;162(4):1139-49.
  Ghadimi MP, Sanzenbacher R, Thiede B, Wenzel J, Jing Q, Plomann M, Borkhardt A, Kabelitz D, Janssen O: Identification of interaction partners of the cytosolic polyproline region of CD95 ligand (CD178). FEBS Lett. 2002 May 22;519(1-3):50-8.
  Jodo S, Pidiyar VJ, Xiao S, Furusaki A, Sharma R, Koike T, Ju ST: Fas ligand (CD178) cytoplasmic tail is a positive regulator of Fas ligand-mediated cytotoxicity. J Immunol. 2005 Apr 15;174(8):4470-4.
  

Target Info

• Gene Symbol: FASLG
• Gene Full Name: Fas ligand
• Alias Symbols: ALPS1B;apoptosis (APO-1) antigen ligand 1;apoptosis antigen ligand;APT1LG1;APTL;CD178;CD95 ligand;CD95L;CD95-L;fas antigen ligand;Fas ligand (TNF superfamily, member 6);FASL;mutant tumor necrosis factor family member 6;TNFSF6;TNLG1A;tumor necrosis factor ligand 1A;tumor necrosis factor ligand superfamily member 6.
• NCBI Gene Id: 356
• Protein Name: Tumor necrosis factor ligand superfamily member 6
• Description of Target: Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells (PubMed:26334989, PubMed:9228058). Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development (PubMed:9228058, PubMed:7528780, PubMed:9427603). Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses (By similarity). TNFRSF6/FAS-mediated apoptosis has also a role in the induction of peripheral tolerance (By similarity). Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis (PubMed:27806260).
• Uniprot ID: P48023
• Protein Accession #: NP_000630.1; NP_001289675.1
• Nucleotide Accession #: NM_000639.2; NM_001302746.1