Size:48 Wells
$328.00
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SKU
OKAA00002-48W
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FAS ELISA Kit (Human) : 48 Wells (OKAA00002-48W)

Catalog#: OKAA00002-48W
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species Reactivity Human
Application ELISA
Kit Detection Method Colorimetric, OD450 nm
Kit Reproducibility The coefficient of variation of both intra-assay and inter-assay were less than 10%.
Kit Component
Components
2 vials human APO-1/FAS Standard lyophilized, 4000 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human APO-1/FAS monoclonal antibody
2 vials Streptavidin-HRP solution,
1 bottle Standard /sample Diluent
1 bottle Biotin-Conjugate antibody Diluent
1 bottle Streptavidin-HRP Diluent
1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
1 vial Substrate Solution
1 vial Stop Solution
1 vial human EGF Standard lyophilized, 20 ng/ml upon reconstitution
1 vial concentrated Biotin-Conjugate anti-human EGF antibody
Additional Information Sample Type: cell culture supernates, serum, and plasma
:: Reagents: REAGENTS
1. Aluminium pouches with a Microwell Plate coated with monoclonal antibody to human APO-1/FAS (8×12)
2. 2 vials human APO-1/FAS Standard lyophilized, 4000 pg/ml upon reconstitution
3. 2 vials concentrated Biotin-Conjugate anti-human APO-1/FAS monoclonal antibody
4. 2 vials Streptavidin-HRP solution,
5. 1 bottle Standard /sample Diluent
6. 1 bottle Biotin-Conjugate antibody Diluent
7. 1 bottle Streptavidin-HRP Diluent
8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
9. 1 vial Substrate Solution
10. 1 vial Stop Solution
11. 3 pieces Adhesive Films
12. package insert
Sample Type cell culture, supernates, serum, and plasma
Sensitivity 31 pg/ml
Predicted Homology Based on Immunogen Sequence Human
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity This assay recognizes both natural and recombinant human APO-1/FAS. The factors listed below were prepared at 50ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
Recombinant human: G-CSF, GM-CSF, IL-1a, IL-1b, IL-2
Recombinant mouse: GM-CSF, TNF-a, MIP-1a, GM-CSF
Other proteins: b FGF acidic, b FGF basic, h PDGF, p PDGF, h TGF-b1
Assay Info Assay Method: Quantitative Sandwich ELISA
Gene Symbol FAS
Official Gene Full Name Fas cell surface death receptor
Alias Symbols ALPS1A, APO-1, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, APT1, CD95, FAS1, FASLG receptor, FASTM, TNFRSF6, Tumor necrosis factor receptor superfamily member 6
NCBI Gene Id 355
Protein Name Tumor necrosis factor receptor superfamily member 6
Description of Target The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform.
Swissprot Id P25445
Protein Accession # NP_000034.1
Nucleotide Accession # NM_000043.5
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express FAS.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express FAS.
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