FAP ELISA Kit (Mouse) (OKCD00760)

Catalog No: OKCD00760 (Formerly GWB-KBBIF6)

Size:96WELLS

Mouse Fap ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	80-99%	78-99%	96-105%	90-98%
  EDTA plasma(n=5)	89-97%	92-105%	86-95%	94-101%
  heparin plasma(n=5)	82-96%	82-104%	95-104%	94-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Activation Protein Alpha (FAPa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Activation Protein Alpha (FAPa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Activation Protein Alpha (FAPa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Fibroblast Activation Protein Alpha (FAPa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	82-105	88
  EDTA plasma(n=5)	80-92	80
  heparin plasma(n=5)	88-95	92

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Activation Protein Alpha (FAPa) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Activation Protein Alpha (FAPa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Fap Microplate: 96 Wells (12 x 8 Well Strips)
  • Fap Lyophilized Standard: 2 x 2 ng
  • 100X Biotinylated Fap Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernatamts and other biological fluids
• Sensitivity: < 0.059 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fibroblast Activation Protein Alpha (FAPa). No significant cross-reactivity or interference between Fibroblast Activation Protein Alpha (FAPa) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Fap
• Gene Full Name: fibroblast activation protein
• Alias Symbols: dipeptidyl peptidase FAP;FAPalpha;fibroblast activation protein alpha;gelatine degradation protease FAP;integral membrane serine protease;post-proline cleaving enzyme;prolyl endopeptidase FAP;seprase;serine integral membrane protease;SIMP;surface-expressed protease.
• NCBI Gene Id: 14089
• Protein Name: Prolyl endopeptidase FAP
• Description of Target: Cell surface glycoprotein serine protease that participates in extracellular matrix degradation and involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences, on substrate such as alpha-2-antiplasmin SERPINF2 and SPRY2. Degrade also gelatin, heat-denatured type I collagen, but not native collagen type I and IV, vibronectin, tenascin, laminin, fibronectin, fibrin or casein. Have also dipeptidyl peptidase activity, exhibiting the ability to hydrolyze the prolyl bond two residues from the N-terminus of synthetic dipeptide substrates provided that the penultimate residue is proline, with a preference for Ala-Pro, Ile-Pro, Gly-Pro, Arg-Pro and Pro-Pro. Natural neuropeptide hormones for dipeptidyl peptidase are the neuropeptide Y (NPY), peptide YY (PYY), substance P (TAC1) and brain natriuretic peptide 32 (NPPB). The plasma membrane form, in association with either DPP4, PLAUR or integrins, is involved in the pericellular proteolysis of the extracellular matrix (ECM), and hence promotes cell adhesion, migration and invasion through the ECM. Plays a role in tissue remodeling during development and wound healing. Participates in the cell invasiveness towards the ECM in malignant melanoma cancers. Enhances tumor growth progression by increasing angiogenesis, collagen fiber degradation and apoptosis and by reducing antitumor response of the immune system. Promotes glioma cell invasion through the brain parenchyma by degrading the proteoglycan brevican. Acts as a tumor suppressor in melanocytic cells through regulation of cell proliferation and survival in a serine protease activity-independent manner.
• Uniprot ID: P97321
• Protein Accession #: NP_032012
• Nucleotide Accession #: NM_007986.3
• Protein Size (# AA): 761