FAM83B ELISA Kit (Human) (OKEH08318)

Catalog No: OKEH08318

Size:96T

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology FAM83B ELISA Kit (Human) (OKEH08318) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FAM83B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FAM83B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FAM83B captured in well.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 113% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=4.6% (n = 20)
  Mean Inter-assay CV%: <=11.0% (n = 20)
• ELISA Kit Component:
  • FAM83B Microplate: 96 Wells (12 x 8 Well strips)
  • FAM83B Lyophilized Standard: 2
  • 100X Biotinylated FAM83B Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Probable proto-oncogene that functions in the epidermal growth factor receptor/EGFR signaling pathway. May activate both the EGFR itself and downstream RAS/MAPK and PI3K/AKT/TOR signaling cascades.
  Subcellular Location: Cytoplasm Membrane
  Unigene: Hs.152423
  SMR: Q5T0W9
  String: 9606.ENSP00000304078
  KEGG: hsa:222584
  Pfam: PF07894
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.24ng/mL
• Specificity: Natural and recombinant Human Protein FAM83B
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: FAM83B
• Gene Full Name: family with sequence similarity 83 member B
• Alias Symbols: C6orf143;protein FAM83B.
• NCBI Gene Id: 222584
• Protein Name: Q5T0W9
• Description of Target: Protein FAM83B
• Uniprot ID: https://www.uniprot.org/uniprot/Q5T0W9
• Protein Accession #: NP_001010872.1
• Nucleotide Accession #: NM_001010872.2