FAM72A ELISA Kit (Human) (OKEH08379)

Catalog No: OKEH08379

Size:96T

Human FAM72A ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology FAM72A ELISA Kit (Human) (OKEH08379) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FAM72A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FAM72A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FAM72A captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 84% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=6.1% (n = 20)
  Mean Inter-assay CV%: <=12.3% (n = 20)
• ELISA Kit Component:
  • FAM72A Microplate: 96 Wells (12 x 8 Well strips)
  • FAM72A Lyophilized Standard: 2
  • 100X Biotinylated FAM72A Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: May play a role in the regulation of cellular reactive oxygen species metabolism. May participate in cell growth regulation.
  Subcellular Location: Cytoplasm Mitochondrion A V5 epitope-tagged construct has been shown to localize to the nucleus (PubMed:18676834). 5-7% (n = 20) of total FAM72A is associated with mitochondria around the nucleus in HEK293 cells (PubMed:21317926).
  Unigene: Hs.339665
  SMR: Q5TYM5
  String: 9606.ENSP00000356096
  KEGG: hsa:729533
  MIM: 614710
  Pfam: PF14976
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 38pg/mL
• Specificity: Natural and recombinant Human Protein FAM72A
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: FAM72A
• Gene Full Name: family with sequence similarity 72 member A
• Alias Symbols: latent membrane protein 1-induced protein;LMP1-induced protein;LMPIP;p17;protein FAM72A;Ugene.
• NCBI Gene Id: 729533
• Protein Name: Q5TYM5
• Description of Target: Protein FAM72A
• Uniprot ID: https://www.uniprot.org/uniprot/Q5TYM5
• Protein Accession #: NP_001116640.1
• Nucleotide Accession #: NM_001123168.2