FAM40B ELISA Kit (Human) (OKEH03299)

Catalog No: OKEH03299

Size:96T

Human FAM40B ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology FAM40B ELISA Kit (Human) (OKEH03299) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human FAM40B antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated FAM40B and incubated. The FAM40B found in the sample or standards competes with the biotinylated FAM40B for limited binding sites on the immobilized anti-Human FAM40B antibody. Excess unbound biotinylated FAM40B and sample or standard FAM40B is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated FAM40B captured in the well and inversely proportional to the amount of FAM40B which was contained in the sample or standard.
• ELISA Kit Range: 0.312-20ng/ml
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86.9%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 7.7% (n = 20)
  Mean Inter-assay CV%: <= 9.0% (n = 20)
• ELISA Kit Component:
  • Anti-FAM40B Microplate: 96 Wells (12 x 8 Well strips)
  • FAM40B Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X FAM40B-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.156 ng/mL
• Specificity: Natural and recombinant Human Protein FAM40B
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: STRIP2
• Gene Full Name: striatin interacting protein 2
• Alias Symbols: FAM40B;family with sequence similarity 40, member B;FAR11 factor arrest 11 homolog B;FAR11B;homolog of yeast FAR11 protein 2;protein FAM40B;striatin-interacting protein 2.
• NCBI Gene Id: 57464
• Protein Name: Q9ULQ0
• Description of Target: Striatin-interacting protein 2
• Uniprot ID: https://www.uniprot.org/uniprot/Q9ULQ0
• Protein Accession #: NP_001127808.1
• Nucleotide Accession #: NM_001134336.1
• Protein Size (# AA): 934
• Molecular Weight: 95 kDa