FAM40A ELISA Kit (Human) (OKEH03301)

Catalog No: OKEH03301

Size:96T

Human FAM40A ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology FAM40A ELISA Kit (Human) (OKEH03301) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human FAM40A antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated FAM40A and incubated. The FAM40A found in the sample or standards competes with the biotinylated FAM40A for limited binding sites on the immobilized anti-Human FAM40A antibody. Excess unbound biotinylated FAM40A and sample or standard FAM40A is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated FAM40A captured in the well and inversely proportional to the amount of FAM40A which was contained in the sample or standard.
• ELISA Kit Range: 0.78-50ng/ml
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 7.6% (n = 20)
  Mean Inter-assay CV%: <= 8.9% (n = 20)
• ELISA Kit Component:
  • Anti-FAM40A Microplate: 96 Wells (12 x 8 Well strips)
  • FAM40A Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X FAM40A-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.396 ng/mL
• Specificity: Natural and recombinant Human Protein FAM40A
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: STRIP1
• Gene Full Name: striatin interacting protein 1
• Alias Symbols: FAM40A;family with sequence similarity 40, member A;FAR11 factor arrest 11 homolog A;FAR11A;homolog of yeast FAR11 protein 1;protein FAM40A;striatin-interacting protein 1.
• NCBI Gene Id: 85369
• Protein Name: Q5VSL9
• Description of Target: Striatin-interacting protein 1
• Uniprot ID: https://www.uniprot.org/uniprot/Q5VSL9
• Protein Accession #: NP_001257697.1
• Nucleotide Accession #: NM_001270768.1
• Protein Size (# AA): 837
• Molecular Weight: 96 kDa