Catalog No: OKEH06558
Size:96T
Price: $600.00
SKU
OKEH06558
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology FAM210A ELISA Kit (Human) (OKEH06558) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-FAM210A antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated FAM210A and incubated. The FAM210A found in the sample or standards competes with the biotinylated FAM210A for limited binding sites on the immobilized anti-FAM210A antibody. Excess unbound biotinylated FAM210A and sample or standard FAM210A is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated FAM210A captured in the well and inversely proportional to the amount of FAM210A which was contained in the sample or standard.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 105%
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Mean Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <= 7.5% (n = 20)
Mean Inter-Assay Precision - <= 8.8% (n = 20)
ELISA Kit Component
ComponentAmount
H3F3C Microplate96 Wells (12 x 8 Well strips)
H3F3C Lyophilized Standard2
100X Biotinylated H3F3C Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.184 ng/mL
SpecificityNatural and recombinant Human Protein FAM210A
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolFAM210A
Gene Full Namefamily with sequence similarity 210 member A
Alias SymbolsC18orf19;HsT2329;protein FAM210A;uncharacterized protein C18orf19.
NCBI Gene Id125228
Protein NameQ96ND0
Description of TargetProtein FAM210A
Uniprot IDhttps://www.uniprot.org/uniprot/Q96ND0
Protein Accession #NP_001257695
Nucleotide Accession #NM_001270766
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