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Catalog No: OKEH01806
Size:96T
Price: $625.00
SKU
OKEH01806
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology FAM151A ELISA Kit (Human) (OKEH01806) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FAM151A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FAM151A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FAM151A captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.1% (n = 20)
Mean Inter-assay CV%: < 11.0% (n = 20)
ELISA Kit Component
ComponentAmount
FAM151A Microplate96 Wells (12 x 8 Well strips)
FAM151A Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated FAM151A Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity34 pg/mL
SpecificityNatural and recombinant Human Protein FAM151A
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolFAM151A
Gene Full Namefamily with sequence similarity 151 member A
Alias SymbolsC1orf179;hyporthetical protein MGC27169;protein FAM151A.
NCBI Gene Id338094
Protein NameQ8WW52
Description of TargetProtein FAM151A
Uniprot IDhttps://www.uniprot.org/uniprot/Q8WW52
Protein Accession #NP_788954.2
Nucleotide Accession #NM_176782.2
Protein Size (# AA)585
Molecular Weight64 kDa
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