FAM132B ELISA Kit (Human) (OKCD09505)

Catalog No: OKCD09505

Size:96WELLS

FAM132B ELISA Kit (Human) (OKCD09505) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Erythroferrone (ERFE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Erythroferrone (ERFE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Erythroferrone (ERFE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Erythroferrone (ERFE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Erythroferrone (ERFE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Erythroferrone (ERFE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • FAM132B Microplate: 96 Wells (12 x 8 Well strips)
  • FAM132B Lyophilized Standard: 2
  • 100X FAM132B HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 0.057ng/mL

Target Info

• Gene Symbol: ERFE
• Gene Full Name: erythroferrone
• Alias Symbols: C1QTNF15;complement C1q tumor necrosis factor-related protein 15;CTRP15;erythroferrone;FAM132B;family with sequence similarity 132, member B;myonectin;protein FAM132B.
• NCBI Gene Id: 151176
• Protein Name: Erythroferrone
• Description of Target: Iron-regulatory hormone that acts as an erythroid regulator after hemorrhage: produced by erythroblasts following blood loss and mediates suppression of hepcidin (HAMP) expression in the liver, thereby promoting increased iron absorption and mobilization from stores. Promotes lipid uptake into adipocytes and hepatocytes via transcriptional up-regulation of genes involved in fatty acid uptake.
• Uniprot ID: Q4G0M1
• Protein Accession #: NP_001278761.1
• Nucleotide Accession #: NM_001291832.1