FAM123B Antibody - middle region (OAAB03558)

Catalog No: OAAB03558

Size:400UL

Western blot analysis of WTX antibody (Center) (Cat. #OAAB03558) in mouse kidney tissue lysates (35ug/lane). WTX (arrow) was detected using the purified Pab.

WTX Antibody (Center) (Cat. #OAAB03558) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WTX Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

WTX Antibody (Center) (Cat. #OAAB03558) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: Flow cytometry, Immunohistochemistry-Paraffin, Western blot
  Function: Regulator of the canonical Wnt signaling pathway. Acts by specifically binding phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), translocating to the cell membrane and interacting with key regulators of the canonical Wnt signaling pathway, such as components of the beta-catenin destruction complex. Acts both as a positive and negative regulator of the Wnt signaling pathway, depending on the context: acts as a positive regulator by promoting LRP6 phosphorylation. Also acts as a negative regulator by acting as a scaffold protein for the beta- catenin destruction complex and promoting stabilization of Axin at the cell membrane. Promotes CTNNB1 ubiquitination and degradation. Involved in kidney development.
  Cellular Location: Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus. Note=Shuttles between nucleus and cytoplasm. Detected in nuclear paraspeckles that are found close to splicing speckles. Translocates to the cell membrane following binding to PtdIns(4,5)P2
  Tissue Location Detected in fetal and adult kidney, brain and spleen.
  
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This WTX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 681-708 amino acids from the Central region of human WTX.
• Purification: This antibody is purified by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: Approximately 2.0 mg/ml. Actual concentration varies with each lot.
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
• Other Applications Image 1 Data: WTX Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Publications:

  Exome Sequencing Reveals AMER1 as a Frequently Mutated Gene in Colorectal Cancer. Clin Cancer Res. 21, 4709-18 (2015). 26071483

• Description:
  View product citations for FAM123B Antibody - middle region (OAAB03558) on CiteAb

Target Info

• Gene Symbol: AMER1
• Gene Full Name: APC membrane recruitment protein 1
• Alias Symbols: adenomatous polyposis coli membrane recruitment 1;APC membrane recruitment protein 1;FAM123B;family with sequence similarity 123B;OSCS;protein FAM123B;RP11-403E24.2;Wilms tumor gene on the X chromosome protein;Wilms tumor on the X;WTX.
• NCBI Gene Id: 139285
• Protein Name: NP_689637.3, Q5JTC6
• Description of Target: APC membrane recruitment protein 1, APC membrane recruitment protein 1 [Homo sapiens]
• Uniprot ID: https://www.uniprot.org/uniprot/Q5JTC6
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_689637.3
• Molecular Weight: 124029