FAM FLICACASPASE-3/7 KIT (OKSA11270)

Catalog No: OKSA11270 (Formerly GWB-Q00443)

Size:25T

Product Info

• Product Format: Kit
• Conjugation: Carboxyfluorescein
• Application: Flow cytometry|Immunofluorescence
• ELISA Kit Component:

  Reagents in the Kit:

  
  

  1 vial of FAM-DEVD-FMK FLICA Reagent - lyophilized
  10X Apoptosis Wash Buffer, 15 mL
  Fixative, 6 mL
  Propidium Iodide, 1 mL
  Hoechst Stain, 1 mL
  

• Additional Information: Fluorophore: FAM (494nm excitation max, 520nm emission max)
• Reconstitution and Storage: 2°C to 8°C
• Specificity: CASPASE-3/7

Target Info

• Description of Target:

  Caspase FLICA™ kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA™ reagent contains a caspase inhibitor sequence linked to a green (Carboxyfluorescein, FAM) fluorescent probe.

  
  

  The Caspase FLICA™ Kits are suitable for cells in suspension, adherent cells, thin tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits.

  
  

  This kit can be used with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates.