FAM FLICA CASPASE-3/7 KIT (OKSA11271)

Catalog No: OKSA11271 (Formerly GWB-Q00444)

Size:100T

Product Info

• Product Format: Kit
• Conjugation: Carboxyfluorescein
• Application: Flow cytometry|Immunofluorescence
• Additional Information:
  Reagents In The Kit
  4 vials of FAM-DEVD-FMK FLICA™ Reagent - lyophilized
  10X Wash Buffer, 60 mL
  Fixative, 6 mL
  Propidium Iodide, 1 mL
  Hoechst Stain, 1 mL
  Test Principle
  Caspase FLICA™ kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA™ reagent contains a caspase inhibitor sequence linked to a green (Carboxyfluorescein, FAM) fluorescent probe.
  The Caspase FLICA™ Kits are suitable for cells in suspension, adherent cells, thin tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits.
  This kit can be used with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates.
  Acknowledgements
  FLICA™ is a trademark of Immunochemistry Technologies, LLC.
  
• Reconstitution and Storage: 2°C to 8°C
• Specificity: CASPASE-3/7
• Application Data: Flow Cytometry, Immunofluorescence

Target Info

• Description of Target: The FAM FLICA™ Caspase 3 & 7 kit uses a quick and easy method to analyze active caspases in apoptotic cells.