Catalog No: OKSA11275 (Formerly GWB-Q00438)
Size:100T
Price: $1,249.00
SKU
OKSA11275
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for FAM FLICA CASPASE-1 KIT (OKSA11275) |
---|
Predicted Species Reactivity | Human |
---|---|
Product Format | Kit |
Conjugation | Carboxyfluorescein |
Application | Flow cytometry|Immunofluorescence |
ELISA Kit Component | -4 vials of FAM-YVAD-FMK Reagent (lyophilized) -10X Wash Buffer (60 mL) -Fixative (6 mL) -Propidium Iodine (1 mL) -Hoechst Stain (1 mL) |
Reconstitution and Storage | 2°C to 8°C |
Predicted Homology Based on Immunogen Sequence | Human |
Reference | References: 1. Hoegen, T. et al. (2011) The NLRP3 Inflammasome Contributes to Brain Injury in Pneumococcal Meningitis and Is Activated through ATP-Dependent Lysosomal Cathepsin B Release. J Immunol. 187: 5440-51. |
Specificity | CASPASE-1 |
Application Data | This kit can be used with a flow cytometer, fluorescence microscope, or a fluorescence plate reader using black microtitre plates. |
Alias Symbols | IL-1 BETA CONVERTING ENZYME |
---|---|
Description of Target | The FAM FLICATM Capase 1 kit uses a target sequence (YVAD) sandwiched between a green fluorescent label, FAM, and FMK to make a quick and flexible method to analyze active capases in apoptotic cells. These kits measure apoptosis by detecting active caspases in whole, living cells. They don't work by using antibodies or an ELISA. Instead, their methodology is based a unique cell-permeable and non-cytotoxic reagent called Fluorochrome Inhibitor of Caspases. These kits are suitable for cells in suspension, adherent cells, thin tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect, and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits. |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
Write Your Own Review