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Catalog No: OKSA11275 (Formerly GWB-Q00438)
Size:100T
Price: $1,249.00
SKU
OKSA11275
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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FAM FLICA CASPASE-1 KIT (OKSA11275)

Datasheets/ManualsPrintable datasheet for FAM FLICA CASPASE-1 KIT (OKSA11275)
Product Info
Predicted Species ReactivityHuman
Product FormatKit
ConjugationCarboxyfluorescein
ApplicationFC, IF, IHC-Fr, IP
ELISA Kit Component-4 vials of FAM-YVAD-FMK Reagent (lyophilized)
-10X Wash Buffer (60 mL)
-Fixative (6 mL)
-Propidium Iodine (1 mL)
-Hoechst Stain (1 mL)
Reconstitution and Storage2°C to 8°C
Predicted Homology Based on Immunogen SequenceHuman
Reference
References:
1. Hoegen, T. et al. (2011) The NLRP3 Inflammasome Contributes to Brain Injury in Pneumococcal Meningitis and Is
Activated through ATP-Dependent Lysosomal Cathepsin B Release. J Immunol. 187: 5440-51.
SpecificityCASPASE-1
Application DataThis kit can be used with a flow cytometer, fluorescence microscope, or a fluorescence plate reader using black microtitre plates.
Alias SymbolsIL-1 BETA CONVERTING ENZYME
Description of TargetThe FAM FLICATM Capase 1 kit uses a target sequence (YVAD) sandwiched between a green fluorescent label, FAM, and FMK to make a quick and flexible method to analyze active capases in apoptotic cells. These kits measure apoptosis by detecting active caspases in whole, living cells. They don't work by using antibodies or an ELISA. Instead, their methodology is based a unique cell-permeable and non-cytotoxic reagent called Fluorochrome Inhibitor of Caspases. These kits are suitable for cells in suspension, adherent cells, thin tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect, and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits.
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