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Catalog No: OKEH08226
Size:96T
Price: $725.00
SKU
OKEH08226
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Linearity
Sample1:21:41:81:16
serum(n=5)109-119% (n = 20)107-116% (n = 20)94-103% (n = 20)108-117% (n = 20)
EDTA plasma(n=5)97-107% (n = 20)103-113% (n = 20)100-112% (n = 20)96-108% (n = 20)
heparin plasma(n=5)107-116% (n = 20)94-106% (n = 20)117-117% (n = 20)113-123% (n = 20)
ELISA Kit PrincipleAviva Systems Biology FAH ELISA Kit (Mouse) (OKEH08226) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FAH has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FAH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FAH captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 117% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=7.1% (n = 20)
Mean Inter-assay CV%: <=10.4% (n = 20)
ELISA Kit Component
ComponentAmount
FAH Microplate96 Wells (12 x 8 Well strips)
FAH Lyophilized Standard2
100X Biotinylated FAH Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
::Unigene: Mm.3798
SMR: P35505
String: 10090.ENSMUSP00000032865
::KEGG: mmu:14085
Pfam: PF01557
Reconstitution and StorageStore as indicated in product manual.
Sensitivity0.048ng/mL
SpecificityNatural and recombinant Mouse Fumarylacetoacetase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolFah
Gene Full Namefumarylacetoacetate hydrolase
Alias Symbolsbeta-diketonase;FAA;fumarylacetoacetase;Fumarylacetoacetate hydrolase;sw;swst.
NCBI Gene Id14085
Protein NameP35505
Description of TargetFumarylacetoacetase
Uniprot IDhttps://www.uniprot.org/uniprot/P35505
Protein Accession #NP_034306.2
Nucleotide Accession #NM_010176.4
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