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Catalog No: OKCD08789
Size:96WELLS
Price: $800.00
SKU
OKCD08789
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Datasheets/ManualsPrintable datasheet for FADS2 ELISA Kit (Human) (OKCD08789)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Desaturase 2 (FADS2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fatty Acid Desaturase 2 (FADS2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fatty Acid Desaturase 2 (FADS2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Fatty Acid Desaturase 2 (FADS2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range31.2-2,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Desaturase 2 (FADS2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Desaturase 2 (FADS2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
FADS2 Microplate96 Wells (12 x 8 Well strips)
FADS2 Lyophilized Standard2
100X FADS2 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids
Sensitivity< 13.4pg/mL
Gene SymbolFADS2
Gene Full Namefatty acid desaturase 2
Alias Symbolsacyl-CoA 6-desaturase;D6D;Delta(6) fatty acid desaturase;delta-6 fatty acid desaturase;delta-6-desaturase;DES6;FADSD6;linoleoyl-CoA desaturase (delta-6-desaturase)-like 2;LLCDL2;SLL0262;TU13.
NCBI Gene Id9415
Protein NameFatty acid desaturase 2
Description of TargetComponent of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18:2n-6) and ALA (18:3n-3) into gamma-linoleic acid (GLA) (18:3n-6) and stearidonic acid (18:4n-3) respectively and other desaturation steps. Highly unsaturated fatty acids (HUFA) play pivotal roles in many biological functions. It catalizes as well the introduction of a cis double bond in palmitate to produce the mono-unsaturated fatty acid sapienate, the most abundant fatty acid in sebum.
Uniprot IDO95864
Protein Accession #NP_001268430.1
Nucleotide Accession #NM_001281501.1
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