FADS2 Antibody - middle region (OAAB02784)

Catalog No: OAAB02784

Size:400ul

FADS2 Antibody (Center) (Cat. #OAAB02784) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the FADS2 antibody detected the FADS2 protein (arrow).

Immunohistochemical analysis of paraffin-embedded H.brain section using FADS2 Antibody (Center)(Cat#OAAB02784). OAAB02784 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

FADS2 Antibody (Center) (Cat. #OAAB02784) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human, Bovine, Xenopus
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IHC-P, FC
  Function: Component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18:2n-6) and ALA (18:3n-3) into gamma- linoleic acid (GLA) (18:3n-6) and stearidonic acid (18:4n-3) respectively and other desaturation steps. Highly unsaturated fatty acids (HUFA) play pivotal roles in many biological functions. It catalizes as well the introduction of a cis double bond in palmitate to produce the mono-unsaturated fatty acid sapienate, the most abundant fatty acid in sebum.
  Cellular Location: Endoplasmic reticulum membrane; Multi-pass membrane protein
  Tissue Location Expressed in a wide array of tissues, highest expression is found in liver followed by brain, lung, heart, and retina. A lower level is found in breast tumor when compared with normal tissues; lowest levels were found in patients with poor prognostic index.
  
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This FADS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-122 amino acids of human FADS2.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Bovine; Xenopus
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: IHC-P: 1:25
  WB: 1:1000
  FC: 1:10~50

Target Info

• Gene Symbol: FADS2
• Alias Symbols: D6D, DES6, TU13, FADSD6, LLCDL2, SLL0262
• NCBI Gene Id: 9415
• Protein Name: Fatty acid desaturase 2
• Description of Target: The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization.
• Uniprot ID: O95864
• Protein Accession #: NP_004256.1
• Molecular Weight: 52259 Da