FADS1 ELISA Kit (Human) (OKEH07448)

Catalog No: OKEH07448

Size:96T

Human FADS1 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology FADS1 ELISA Kit (Human) (OKEH07448) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FADS1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FADS1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FADS1 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 108% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=5.1% (n = 20)
  Mean Inter-assay CV%: <=7.2% (n = 20)
• ELISA Kit Component:
  • FADS1 Microplate: 96 Wells (12 x 8 Well strips)
  • FADS1 Lyophilized Standard: 2
  • 100X Biotinylated FADS1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Isoform 2: Does not exhibit any catalytic activity toward 20:3n-6, but it may enhance FADS2 activity.
  Subcellular Location: Isoform 2 Endoplasmic reticulum membrane Multi-pass membrane protein
  Unigene: Hs.503546
  SMR: O60427
  String: 9606.ENSP00000322229
  KEGG: hsa:3992
  MIM: 606148
  Pfam: PF00173
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.091ng/mL
• Specificity: Natural and recombinant Human Acyl-CoA (8-3)-desaturase
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: FADS1
• Gene Full Name: fatty acid desaturase 1
• Alias Symbols: acyl-CoA (8-3)-desaturase;D5D;delta(5) desaturase;Delta(5) fatty acid desaturase;delta-5 fatty acid desaturase;FADS6;FADSD5;linoleoyl-CoA desaturase (delta-6-desaturase)-like 1;LLCDL1;TU12.
• NCBI Gene Id: 3992
• Protein Name: O60427
• Description of Target: Fatty acid desaturase 1
• Uniprot ID: https://www.uniprot.org/uniprot/O60427
• Protein Accession #: NP_037534.3
• Nucleotide Accession #: NM_013402.4