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Catalog No: OKCD09235
Size:96WELLS
Price: $800.00
SKU
OKCD09235
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Datasheets/ManualsPrintable datasheet for FADD ELISA Kit (Human) (OKCD09235)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fas Associating Death Domain Containing Protein (FADD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fas Associating Death Domain Containing Protein (FADD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fas Associating Death Domain Containing Protein (FADD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Fas Associating Death Domain Containing Protein (FADD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fas Associating Death Domain Containing Protein (FADD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fas Associating Death Domain Containing Protein (FADD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
FADD Microplate96 Wells (12 x 8 Well strips)
FADD Lyophilized Standard2
100X FADD HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 0.065ng/mL
Gene SymbolFADD
Gene Full NameFas associated via death domain
Alias SymbolsFas (TNFRSF6)-associated via death domain;FAS-associated death domain protein;Fas-associating death domain-containing protein;Fas-associating protein with death domain;GIG3;growth-inhibiting gene 3 protein;Mediator of receptor induced toxicity;mediator of receptor-induced toxicity;MORT1;Protein FADD.
NCBI Gene Id8772
Protein NameFAS-associated death domain protein
Description of TargetApoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis. Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling.
Uniprot IDQ13158
Protein Accession #NP_003815.1
Nucleotide Accession #NM_003824.3
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