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Datasheets/Manuals | Printable datasheet for anti-Factor-IX antibody - OAPA00105 |
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Product Format | Sterile filtered white lyophilized (freeze-dried) powder |
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Clonality | Monoclonal |
Clone | NYRhFIX. |
Isotype | Mouse IgG. |
Host | Mouse |
Application | WB, IHC |
Additional Information | Titer: By direct ELISA, 1:10,000 dilution will yield 0.5 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories). |
Reconstitution and Storage | Reconstitute with H20 to get a 1 mg/ml concentration. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. In lyophilized form, for long periods, store at 4C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20C. |
Immunogen | The immunogen for anti-Factor-IX antibody: human Factor-IX. |
Purification | Protein A. |
Concentration | 1 mg/ml in PBS (after reconstitution) |
Application Info | Direct ELISA, Western Blot, Imunohistochemistry. For W.B. use 1 ug/ml of antibody. |
Gene Symbol | Factor-IX |
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Alias Symbols | FIX, P19, PTC, HEMB, THPH8, F9 p22 |
NCBI Gene Id | 2158 |
Protein Name | Coagulation factor IX |
Description of Target | Factor IX is a glycoprotein, which is synthesized in the liver. The domain structure of factor IX is similar to that of the other vitamin K dependent coagulation factors. The NH2-terminal region contains 12 g-carboxyglutamic acid (gla) residues, which facilitate the calcium dependent binding of factor IX to negatively charged phospholipid surfaces. Two domains which are homologous to epidermal growth factor (EGF) span the region between the NH2-terminal gla domain and the activation peptide (Ala-146 to Arg-180). Factor IX is activated by either factor XIa or the factor VIIa/tissue factor/phospholipid complex. Cleavage at site A yields the intermediate IXa, which is subsequently converted to the fully active form IXab by cleavage at site B. The NH2-terminal light chain (GLA and EGF domains) remains covalently attached to the COOH-terminal heavy chain by a disulfide bond. The serine protease catalytic triad (Ser-365, His 221, Asp-269) is located in the heavy chain. Factor IXab is the catalytic component of the "intrinsic factor Xase complex" (factor VIIIa/IXa/Ca2+/phospholipid) which proteolytically activates factor X to factor Xa. |
Uniprot ID | P00740 |
Protein Accession # | NP_000124.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Factor-IX Antibody (OAPA00105)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "".
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How long will it take to receive "Factor-IX Antibody (OAPA00105)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "Factor-IX Antibody (OAPA00105)" provided in?
This item is provided in "Sterile filtered white lyophilized (freeze-dried) powder".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Factor-IX Antibody (OAPA00105)"?
This target may also be called "FIX, P19, PTC, HEMB, THPH8, F9 p22" in publications.
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What is the shipping cost for "Factor-IX Antibody (OAPA00105)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Factor-IX Antibody (OAPA00105)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Factor-IX Antibody (OAPA00105)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Factor-IX Antibody (OAPA00105)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "FACTOR-IX"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "FACTOR-IX"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "FACTOR-IX"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "FACTOR-IX"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "FACTOR-IX"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "FACTOR-IX"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.