FABP7 ELISA Kit (Human) (OKCD02536)

Catalog No: OKCD02536

Size:96WELLS

Human FABP7 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	93-101%	99-105%	86-97%	91-103%
  EDTA Plasma (n=5)	78-101%	91-105%	79-105%	99-105%
  Heparin Plasma (n=5)	79-101%	91-99%	80-97%	80-90%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Binding Protein 7, Brain (FABP7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fatty Acid Binding Protein 7, Brain (FABP7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fatty Acid Binding Protein 7, Brain (FABP7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Fatty Acid Binding Protein 7, Brain (FABP7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.47-30ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	79-95	91
  EDTA Plasma (n=5)	80-103	93
  Heparin Plasma(n=5)	89-96	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Binding Protein 7, Brain (FABP7) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Binding Protein 7, Brain (FABP7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-FABP7 Microplate: 96 Wells (12 x 8 Well strips)
  • FABP7 Lyophilized Standard: 2
  • 100X Biotinylated FABP7 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids
• Sensitivity: < 0.19 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fatty Acid Binding Protein 7, Brain (FABP7). No significant cross-reactivity or interference between Fatty Acid Binding Protein 7, Brain (FABP7) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: FABP7
• Gene Full Name: fatty acid binding protein 7
• Alias Symbols: B-FABP;BLBP;brain lipid-binding protein;brain-type fatty acid-binding protein;FABPB;Fatty acid-binding protein 7;fatty acid-binding protein, brain;hypothetical protein DKFZp547J2313;Mammary-derived growth inhibitor related;mammary-derived growth inhibitor-related;MRG.
• NCBI Gene Id: 2173
• Protein Name: Fatty acid-binding protein, brain
• Description of Target: B-FABP could be involved in the transport of a so far unknown hydrophobic ligand with potential morphogenic activity during CNS development. It is required for the establishment of the radial glial fiber system in developing brain, a system that is necessary for the migration of immature neurons to establish cortical layers (By similarity).
• Uniprot ID: O15540
• Protein Accession #: NP_001305968.1
• Nucleotide Accession #: NM_001319039.1
• Protein Size (# AA): 132