FABP6 ELISA Kit (Rat) (OKCD02427)

Catalog No: OKCD02427

Size:96WELLS

Rat FABP6 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	92-99%	91-98%	79-101%	99-105%
  EDTA Plasma (n=5)	99-105%	84-94%	89-103%	78-96%
  Heparin Plasma (n=5)	99-105%	80-97%	79-94%	84-98%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Binding Protein 6, Ileal (FABP6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fatty Acid Binding Protein 6, Ileal (FABP6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fatty Acid Binding Protein 6, Ileal (FABP6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Fatty Acid Binding Protein 6, Ileal (FABP6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	94-102	98
  EDTA Plasma (n=5)	87-101	91
  Heparin Plasma(n=5)	83-97	91

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Binding Protein 6, Ileal (FABP6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Binding Protein 6, Ileal (FABP6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-FABP6 Microplate: 96 Wells (12 x 8 Well strips)
  • FABP6 Lyophilized Standard: 2
  • 100X Biotinylated FABP6 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 27 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Fatty Acid Binding Protein 6, Ileal (FABP6). No significant cross-reactivity or interference between Fatty Acid Binding Protein 6, Ileal (FABP6) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Fabp6
• Gene Full Name: fatty acid binding protein 6
• Alias Symbols: 14 kDa bile acid-binding protein;Fatty acid binding protein 6 (bile acid-binding protein);fatty acid binding protein 6, ileal;Fatty acid-binding protein 6;gastrotropin;GT;I-15P;IBABP;I-BABP;ILBP;ileal lipid-binding protein;intestinal 15 kDa protein;strain SHR/OlaIpcv fatty acid binding protein 6 (Fabp6) pseudogene.
• NCBI Gene Id: 25440
• Protein Name: Gastrotropin
• Description of Target: Binds to bile acids and is involved in enterohepatic bile acid metabolism. Required for efficient apical to basolateral transport of conjugated bile acids in ileal enterocytes (By similarity). Stimulates gastric acid and pepsinogen secretion (By similarity).
• Uniprot ID: P80020
• Protein Accession #: NP_058794.1
• Nucleotide Accession #: NM_017098.1
• Protein Size (# AA): 128