FABP4 ELISA Kit (Mouse) (OKCD07599)

Catalog No: OKCD07599

Size:96WELLS

FABP4 ELISA Kit (Mouse) (OKCD07599) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Binding Protein 4 (FABP4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fatty Acid Binding Protein 4 (FABP4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fatty Acid Binding Protein 4 (FABP4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Fatty Acid Binding Protein 4 (FABP4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Binding Protein 4 (FABP4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Binding Protein 4 (FABP4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • FABP4 Microplate: 96 Wells (12 x 8 Well strips)
  • FABP4 Lyophilized Standard: 2
  • 100X FABP4 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 13.8pg/mL

Target Info

• Gene Symbol: Fabp4
• Gene Full Name: fatty acid binding protein 4, adipocyte
• Alias Symbols: 3T3-L1 lipid-binding protein;422/a;422/aP2;A;adipocyte lipid-binding protein;adipocyte protein aP2;adipocyte-type fatty acid-binding protein;AFABP;ALB;ALBP;ALBP/Ap2;Ap2;fatty acid-binding protein 4;fatty acid-binding protein, adipocyte;L;Lbpl;myelin P2 protein homolog;P15;P2 adipocyte protein;protein 422.
• NCBI Gene Id: 11770
• Protein Name: Fatty acid-binding protein, adipocyte
• Description of Target: Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.
• Uniprot ID: P04117
• Protein Accession #: NP_077717.1
• Nucleotide Accession #: NM_024406.2