Catalog No: OKCD07243
Size:96WELLS
Price: $750.00
SKU
OKCD07243
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Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heart-type Fatty Acid Binding Protein (H-FABP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heart-type Fatty Acid Binding Protein (H-FABP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heart-type Fatty Acid Binding Protein (H-FABP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Heart-type Fatty Acid Binding Protein (H-FABP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.625-40ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heart-type Fatty Acid Binding Protein (H-FABP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heart-type Fatty Acid Binding Protein (H-FABP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
FABP3 Microplate96 Wells (12 x 8 Well strips)
FABP3 Lyophilized Standard2
100X FABP3 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 0.251ng/mL
Gene SymbolFABP3
Gene Full Namefatty acid binding protein 3
Alias Symbolsepididymis secretory sperm binding protein;FABP11;fatty acid binding protein 11;fatty acid binding protein 3, muscle and heart;Fatty acid-binding protein 3;fatty acid-binding protein, heart;heart-type fatty acid-binding protein;H-FABP;mammary-derived growth inhibitor;MDGI;M-FABP;muscle fatty acid-binding protein;O-FABP.
NCBI Gene Id2170
Protein NameFatty acid-binding protein, heart
Description of TargetFABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.
Uniprot IDP05413
Protein Accession #NP_004093.1
Nucleotide Accession #NM_004102.4
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