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Catalog No: OKEH00897
Size:96T
Price: $525.00
SKU
OKEH00897
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology FABP3 ELISA Kit (Human) (OKEH00897) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FABP3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FABP3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FABP3 captured in well.
ELISA Kit Range0.47-30ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.7% (n = 20)
Mean Inter-assay CV%: < 7.2% (n = 20)
ELISA Kit Component
ComponentAmount
FABP3 Microplate96 Wells (12 x 8 Well strips)
FABP3 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated FABP3 Detector Antibody1 x 120 ul
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.24 ng/mL
SpecificityNatural and recombinant Human Fatty acid-binding protein, heart
Publications

Impaired Relaxation and Reduced Lusitropic Reserve in Atrial Myocardium in the Obese Patients. Front Cardiovasc Med. 8, 739907 (2021). 34778401

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Description
Gene SymbolFABP3
Gene Full Namefatty acid binding protein 3
Alias Symbolsepididymis secretory sperm binding protein;FABP11;fatty acid binding protein 11;fatty acid binding protein 3, muscle and heart;Fatty acid-binding protein 3;fatty acid-binding protein, heart;heart-type fatty acid-binding protein;H-FABP;mammary-derived growth inhibitor;MDGI;M-FABP;muscle fatty acid-binding protein;O-FABP.
NCBI Gene Id2170
Protein NameP05413
Description of TargetFatty acid-binding protein, heart
Uniprot IDhttps://www.uniprot.org/uniprot/P05413
Protein Accession #NP_004093.1
Nucleotide Accession #NM_004102.3
Protein Size (# AA)133
Molecular Weight14 kDa
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