FABP3 ELISA Kit (Human) : 96 Wells (OKEH00897)

Catalog No: OKEH00897

Size:96 Wells

Human FABP3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology FABP3 ELISA Kit (Human) (OKEH00897) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FABP3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FABP3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FABP3 captured in well.
  
• ELISA Kit Range: 0.47 - 30ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.7% (n = 20)
  Mean Inter-assay CV%: < 7.2% (n = 20)
• ELISA Kit Component:
  • FABP3 Microplate: 96 Wells (12 x 8 Well strips)
  • FABP3 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated FABP3 Detector Antibody: 1 x 120 ul
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.24 ng/mL
• Specificity: Natural and recombinant Human Fatty acid-binding protein, heart
• Publications:

  Impaired Relaxation and Reduced Lusitropic Reserve in Atrial Myocardium in the Obese Patients. Front Cardiovasc Med. 8, 739907 (2021). 34778401

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: FABP3
• Gene Full Name: fatty acid binding protein 3
• Alias Symbols: MDGI, FABP11, H-FABP, M-FABP, O-FABP
• NCBI Gene Id: 2170
• Protein Name: Fatty acid-binding protein, heart
• Description of Target: The intracellular fatty acid-binding proteins (FABPs) belongs to a multigene family. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is a candidate tumor suppressor gene for human breast cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
• Uniprot ID: P05413
• Protein Accession #: NP_004093.1
• Nucleotide Accession #: NM_004102.3
• Protein Size (# AA): 133
• Molecular Weight: 14 kDa