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Catalog No: OKEH01560
Size:96T
Price: $425.00
SKU
OKEH01560
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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FABP1 ELISA Kit (Human) : 96 Wells (OKEH01560)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology FABP1 ELISA Kit (Human) (OKEH01560) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FABP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FABP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FABP1 captured in well.
ELISA Kit Range6.25-400ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.4% (n = 20)
Mean Inter-assay CV%: < 6.8% (n = 20)
ELISA Kit Component
ComponentAmount
FABP1 Microplate96 Wells (12 x 8 Well strips)
FABP1 Lyophilized Standard2
100X Biotinylated FABP1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity3.12 ng/mL
SpecificityNatural and recombinant Human Fatty acid-binding protein, liver
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolFABP1
Gene Full Namefatty acid binding protein 1
Alias SymbolsFABPL;fatty acid binding protein 1, liver;Fatty acid-binding protein 1;fatty acid-binding protein, liver;L-FABP;liver-type fatty acid-binding protein.
NCBI Gene Id2168
Protein NameP07148
Description of TargetFatty acid-binding protein, liver
Uniprot IDhttps://www.uniprot.org/uniprot/P07148
Protein Accession #NP_001434.1
Nucleotide Accession #NM_001443.2
Protein Size (# AA)127
Molecular Weight14 kDa
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