FAAH ELISA Kit (Mouse) (OKEH08277)

Catalog No: OKEH08277

Size:96T

Mouse FAAH ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology FAAH ELISA Kit (Mouse) (OKEH08277) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FAAH has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FAAH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FAAH captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 114% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=4.1% (n = 20)
  Mean Inter-assay CV%: <=9.2% (n = 20)
• ELISA Kit Component:
  • FAAH Microplate: 96 Wells (12 x 8 Well strips)
  • FAAH Lyophilized Standard: 2
  • 100X Biotinylated FAAH Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates.
  Subcellular Location: Endoplasmic reticulum membrane Single-pass membrane protein Golgi apparatus membrane Single-pass membrane protein Seems to be associated with the endoplasmic reticulum and/or Golgi apparatus.
  Unigene: Mm.256025
  SMR: O08914
  String: 10090.ENSMUSP00000041543
  KEGG: mmu:14073
  Pfam: PF01425
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 39.4pg/mL
• Specificity: Natural and recombinant Mouse Fatty-acid amide hydrolase 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Faah
• Gene Full Name: fatty acid amide hydrolase
• Alias Symbols: amide hydrolase;anandamide amidohydrolase 1;AW412498;fatty acid amide hydrolase protein;fatty acid ester hydrolase;fatty-acid amide hydrolase 1;oleamide hydrolase 1.
• NCBI Gene Id: 14073
• Protein Name: O08914
• Description of Target: Fatty-acid amide hydrolase 1
• Uniprot ID: https://www.uniprot.org/uniprot/O08914
• Protein Accession #: NP_034303.3
• Nucleotide Accession #: NM_010173.4