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Catalog No: OKEH03877
Size:96T
Price: $625.00
SKU
OKEH03877
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityPorcine, Sus scrofa
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology F8 ELISA Kit (Pig) (OKEH03877) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for F8 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for F8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample F8 captured in well.
ELISA Kit Range1.56-100ng/mL
ELISA Kit Component
ComponentAmount
Anti-F8 Microplate96 Wells (12 x 8 Well strips)
F8 Lyophilized Standard2
100X Biotinylated F8 Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.48 ng/mL
SpecificityNatural and recombinant Pig Coagulation factor VIII
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolF8
Gene Full Namecoagulation factor VIII
Alias Symbolscoagulation factor VIII;coagulation factor VIII, procoagulant component (hemophilia A);factor VIII;procoagulant component.
NCBI Gene Id397339
Protein NameP12263
Description of TargetCoagulation factor VIII
Uniprot IDhttps://www.uniprot.org/uniprot/P12263
Protein Accession #NP_999332.1
Nucleotide Accession #NM_214167.1
Protein Size (# AA)2133
Molecular Weight239 kDa
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