Catalog No: OKEH01339
Size:96T
Price: $525.00
SKU
OKEH01339
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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F7 ELISA Kit (Human) : 96 Wells (OKEH01339)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology F7 ELISA Kit (Human) (OKEH01339) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for F7 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for F7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample F7 captured in well.
ELISA Kit Range15.6-1000ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.2% (n = 20)
Mean Inter-assay CV%: < 9.7% (n = 20)
ELISA Kit Component
ComponentAmount
F7 Microplate96 Wells (12 x 8 Well strips)
F7 Lyophilized Standard2
100X Biotinylated F7 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity7.8 ng/mL
SpecificityNatural and recombinant Human Coagulation factor VII
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolF7
Gene Full Namecoagulation factor VII
Alias Symbolscoagulation factor VII;coagulation factor VII (serum prothrombin conversion accelerator);eptacog alfa;FVII coagulation protein;proconvertin;Serum prothrombin conversion accelerator;SPCA.
NCBI Gene Id2155
Protein NameP08709
Description of TargetCoagulation factor VII
Uniprot IDhttps://www.uniprot.org/uniprot/P08709
Protein Accession #NP_000122.1
Nucleotide Accession #NM_000131.4
Protein Size (# AA)466
Molecular Weight52 kDa
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