F5 ELISA Kit (Human) (OKCD06653)

Catalog No: OKCD06653

Size:96WELLS

F5 ELISA Kit (Human) (OKCD06653) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor V (F5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coagulation Factor V (F5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coagulation Factor V (F5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Coagulation Factor V (F5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 125-8,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coagulation Factor V (F5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coagulation Factor V (F5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • F5 Microplate: 96 Wells (12 x 8 Well strips)
  • F5 Lyophilized Standard: 2
  • 100X F5 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Plasma
• Sensitivity: < 61pg/mL

Target Info

• Gene Symbol: F5
• Gene Full Name: coagulation factor V
• Alias Symbols: activated protein c cofactor;coagulation factor V;coagulation factor V (proaccelerin, labile factor);coagulation factor V jinjiang A2 domain;factor V Leiden;FVL;PCCF;Proaccelerin, labile factor;RPRGL1;THPH2.
• NCBI Gene Id: 2153
• Protein Name: Coagulation factor V
• Description of Target: Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin.
• Uniprot ID: P12259
• Protein Accession #: NP_000121.2
• Nucleotide Accession #: NM_000130.4