F3 ELISA Kit (Rat) (OKEH04104)

Catalog No: OKEH04104

Size:96T

Rat F3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology F3 ELISA Kit (Rat) (OKEH04104) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for F3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for F3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample F3 captured in the well.
• ELISA Kit Range: 15.6-1000pg/mL
• ELISA Kit Component:
  • Anti-F3 Microplate: 96 Wells (12 x 8 Well Strips)
  • F3 Lyophilized Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • F3 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 7.87 pg/mL
• Specificity: Natural and recombinant Rat Tissue factor
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: F3
• Gene Full Name: coagulation factor III, tissue factor
• Alias Symbols: Coagulation factor III.
• NCBI Gene Id: 25584
• Protein Name: P42533
• Description of Target: Tissue factor
• Uniprot ID: https://www.uniprot.org/uniprot/P42533
• Protein Accession #: NP_037189.2
• Nucleotide Accession #: NM_013057.2
• Protein Size (# AA): 295
• Molecular Weight: 33 kDa