F3 ELISA Kit (Rat) (OKCA02255)

Catalog No: OKCA02255

Size:96 Wells

Rat F3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Sample Type	Dilution	Mean (%)	Range (%)
  Serum (n=4)	Neat	102	91-106
  Serum (n=4)	1:2	90	85-96
  Serum (n=4)	1:4	93	89-100
  Serum (n=4)	1:8	99	93-108

• ELISA Kit Principle: Aviva Systems Biology F3 ELISA Kit (Rat) (OKCA02255) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for F3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for F3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample F3 captured in well.
• ELISA Kit Range: 31.25 pg/mL-2,000 pg/mL
• ELISA Kit Recovery:

  Sample Type	Mean (%)	Range (%)
  Serum (n=5)	88	84-94
  EDTA plasma (n=4)	103	95-107

• ELISA Kit Reproducibility: Mean Intra-Assay Variation <10% CV (n=20)
  Mean Inter-Assay Variation <12% CV (n=8)
• ELISA Kit Component:
  • Anti-F3 Microplate: 96 Wells (12 x 8 Well strips)
  • F3 Lyophilized Standard: 2 x 2 ng
  • 100X Biotinylated F3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Urine, Cell Culture Supernates, Tissue Homogenates, Cell Lysates
• Sensitivity: 7.8 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: F3
• NCBI Gene Id: 100009127
• Protein Name: Tissue factor
• Description of Target: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
• Uniprot ID: P24055
• Protein Accession #: NP_001075760
• Nucleotide Accession #: NM_001082291