F3 Chemi-Luminescent ELISA Kit (Human) (OKCD03937)

Catalog No: OKCD03937

Size:96WELLS

Human F3 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	89-97%	94-102%	87-105%	95-103%
  EDTA Plasma (n=5)	92-105%	79-95%	82-97%	83-91%
  Heparin Plasma (n=5)	78-98%	90-103%	96-104%	93-104%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Tissue Factor (TF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Tissue Factor (TF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Tissue Factor (TF) level in the sample or standard.
• ELISA Kit Range: 2.74-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	80-102	85
  EDTA Plasma (n=5)	92-103	99
  Heparin Plasma (n=5)	78-97	84

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tissue Factor (TF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tissue Factor (TF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • F3 Microplate: 96 Wells (12 x 8 Well strips)
  • F3 Lyophilized Standard: 2
  • 100X F3 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 31.25 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: F3
• Gene Full Name: coagulation factor III, tissue factor
• Alias Symbols: CD142;Coagulation factor III;coagulation factor III (thromboplastin, tissue factor);TF;TFA;Thromboplastin;tissue factor.
• NCBI Gene Id: 2152
• Protein Name: Tissue factor
• Description of Target: Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
• Uniprot ID: P13726
• Protein Accession #: NP_001171567
• Nucleotide Accession #: NM_001178096