F2R ELISA Kit (Mouse) (OKEH03227)

Catalog No: OKEH03227

Size:96T

Mouse F2R ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology F2R ELISA Kit (Mouse) (OKEH03227) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for F2r has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for F2r is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample F2r captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: <= 6.5%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 6.5% (n = 20)
  Mean Inter-assay CV%: <= 8.0% (n = 20)
• ELISA Kit Component:
  • Anti-F2r Microplate: 96 Wells (12 x 8 Well strips)
  • F2r Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated F2r Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.166 ng/mL
• Specificity: Natural and recombinant Mouse Proteinase-activated receptor 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: F2r
• Gene Full Name: coagulation factor II (thrombin) receptor
• Alias Symbols: AI482343;Cf2;Cf2r;P;Par1;proteinase-activated receptor 1;Th;Thrombin receptor;ThrR.
• NCBI Gene Id: 14062
• Protein Name: P30558
• Description of Target: Proteinase-activated receptor 1
• Uniprot ID: https://www.uniprot.org/uniprot/P30558
• Protein Accession #: NP_034299.2
• Nucleotide Accession #: NM_010169.3
• Protein Size (# AA): 430
• Molecular Weight: 48 kDa