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Catalog No: OKCD01330
Size:96WELLS
Price: $675.00
SKU
OKCD01330
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F2 High Sensitivity ELISA Kit (Human) (OKCD01330)

Datasheets/ManualsPrintable datasheet for F2 High Sensitivity ELISA Kit (Human) (OKCD01330)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
EDTA Plasma (n=5)91-101%85-99%82-89%87-94%
Heparin Plasma (n=5)97-105%79-98%90-104%78-101%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to F1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to F2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain F1+2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of F1+2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.11-80pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
EDTA Plasma (n=5)90-10193
Heparin Plasma (n=5)89-9693
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Prothrombin Fragment 1+2 (F1+2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Prothrombin Fragment 1+2 (F1+2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-F2 Microplate96 Wells (12 x 8 Well Strips)
F2 Lyophilized Standard2
100X Biotinylated F2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypePlasma
Sensitivity0.08 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Prothrombin Fragment 1+2 (F1+2). No significant cross-reactivity or interference between Prothrombin Fragment 1+2 (F1+2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolF2
Gene Full Namecoagulation factor II, thrombin
Alias SymbolsCoagulation factor II;prepro-coagulation factor II;prothrombin;prothrombin B-chain;PT;RPRGL2;THPH1;thrombin factor II.
NCBI Gene Id2147
Protein NameProthrombin
Description of TargetThrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.
Uniprot IDP00734
Protein Accession #NP_000497.1
Nucleotide Accession #NM_000506.4
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