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Catalog No: OKCD02529
Size:96WELLS
Price: $625.00
SKU
OKCD02529
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Datasheets/ManualsPrintable datasheet for F2 ELISA Kit (Mouse) (OKCD02529)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
sodium citrate plasma(n=5)87-98%89-101%80-101%90-104%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor II (F2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coagulation Factor II (F2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coagulation Factor II (F2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Coagulation Factor II (F2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range125-8,000ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
sodium citrate plasma(n=5)78-10299
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coagulation Factor II (F2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coagulation Factor II (F2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-F2 Microplate96 Wells (12 x 8 Well strips)
F2 Lyophilized Standard2
100X Biotinylated F2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypePlasma
Sensitivity0.59 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Coagulation Factor II (F2). No significant cross-reactivity or interference between Coagulation Factor II (F2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolF2
Gene Full Namecoagulation factor II
Alias SymbolsCf2;Cf-2;Coagulation factor II;F;FII;prot;prothrombin;thro;thrombin.
NCBI Gene Id14061
Protein NameProthrombin
Description of TargetThrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing (By similarity).
Uniprot IDP19221
Protein Accession #NP_034298.1
Nucleotide Accession #NM_010168.3
Protein Size (# AA)618
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