F2 Chemi-Luminescent ELISA Kit (Human) (OKCD03443)

Catalog No: OKCD03443

Size:96WELLS

Human F2 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	81-101%	90-98%	86-95%	88-101%
  EDTA Plasma (n=5)	98-105%	94-102%	96-105%	95-103%
  Heparin Plasma (n=5)	92-101%	78-97%	85-92%	78-89%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Prothrombin Fragment 1+2 (F1+2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Prothrombin Fragment 1+2 (F1+2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Prothrombin Fragment 1+2 (F1+2) level in the sample or standard.
• ELISA Kit Range: 2.74-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	78-102	85
  EDTA Plasma (n=5)	84-101	90
  Heparin Plasma (n=5)	85-92	89

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prothrombin Fragment 1+2 (F1+2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prothrombin Fragment 1+2 (F1+2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • F2 Microplate: 96 Wells (12 x 8 Well strips)
  • F2 Lyophilized Standard: 2
  • 100X F2 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
• Sensitivity: 31.25 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: F2
• Gene Full Name: coagulation factor II, thrombin
• Alias Symbols: Coagulation factor II;prepro-coagulation factor II;prothrombin;prothrombin B-chain;PT;RPRGL2;THPH1;thrombin factor II.
• NCBI Gene Id: 2147
• Protein Name: Prothrombin
• Description of Target: Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.
• Uniprot ID: P00734
• Protein Accession #: NP_000497
• Nucleotide Accession #: NM_000506